Preparation of P(3HB) microparticles modified with type I collagen with antiseptic properties and investigation of their characteristics

https://doi.org/10.1007/s00289-025-06203-6

The aim of this study was to develop polymer microparticles conjugated with type I collagen and loaded with the antiseptic brilliant green (tetraethyl 4,4-diaminotriphenylmethane oxalate) for wound treatment and healing of skin lesions. Microparticles loaded with brilliant green (BG) were synthesized by the emulsification method with the achieved encapsulation efficiency of 95.5%. Collagen was covalently attached to the surface of the polymer microparticles. The average size of the microparticles with deposited antiseptic was 57.2 ± 0.2 μm, and that of the microparticles with collagen was 58.9 ± 0.4 μm. Their respective zeta potential values were − 27.3 mV and − 31.7 mV. Scanning electron microscopy (SEM) showed uniform spherical morphology of the particles. The results of the in vitro release study demonstrated a sustained release of BG for more than 600 h. Moreover, surface modification of the microparticles with type I collagen significantly improved cell adhesion and biocompatibility of the local antiseptic delivery system in NIH/3T3 mouse fibroblast cultures.