Phytogenic Synthesis of Ag Bionano-Antibiotics Against ESKAPE Drug Resistant Communities in Krasnoyarsk, Siberia

Syed, Baker; Prudnikova, Svetlana, V; Perianoval, Olga, V; Zharkov, Sergey M.; Kuzmin, Andrey; Chouhan, Raghuraj Singh; Potkina, Nadezhda K.; Khohlova, Olga Y.; Lobova, Tatiana, I; Singh, Manoj Source: JOURNAL OF CLUSTER SCIENCE, 30 (3):589-597; 10.1007/s10876-019-01518-7 MAY 2019

The present study reports synthesis of silver nano-antibiotics from aqueous extract of H. dissectum. The nano-antibiotics were characterized using UV-visible spectra which displayed maximum absorbance at 430nm. The role of phytocomponents as reducing agent and stabilization of silver nano-antibiotics was depicted with Fourier-transform infrared spectroscopy which revealed presence of hydroxyl and carboxyl groups associated with silver nano-antibiotics. The crystalline nature was studied with X-ray diffraction which showed Bragg's intensities at 2 angle which denoted (101), (111), (200), (220), (311) planes. The morphological characteristics were studied using Transmission Electron Microscopy to reveal the size ranging from 5 to 50nm. The bactericidal activity of nano-antibiotics was studied via well diffusion and minimal inhibitory concentration assay against members of drug-resistant communities bearing resistant to more than ten standard antibiotics. Nano-antibiotics displayed highest activity against Ps. aeruginosa strain 55 and Methicillin-resistant Staphylococcus aureus with 16mm zone of inhibition followed by Kl. pneumoniae strain 104 and E. coli strain 55. Moderate activity was observed against Ac. baumannii strains with 12mm zone of inhibition. The obtained results envision the role of plant mediated nano-antibiotics as one of the best suited alternatives towards combating drug resistant pathogens.